site stats

Ctsk-cre小鼠

Web“找小鼠,上红鼠!”赛业“红鼠”已包含16000品系基因敲除、条件性敲..... 更多 ›› Web004781 B6.129P2- Lyz2 tm1(cre)Ifo /J The LysMcre knock-in/knock-out allele has a nuclear-localized Cre recombinase inserted into the first coding ATG of the lysozyme 2 gene ( Lyz2 ); both abolishing endogenous Lyz2 gene function and placing NLS-Cre expression under the control of the endogenous Lyz2 promoter/enhancer elements.

小鼠研究院丨层层解析条件性基因敲除的“时空开关”—— Cre-loxP …

WebMay 17, 2024 · Lysm 是溶菌酶lysozyme的缩写,而Cre是一种识别特异位点的基因重组酶,Lysm cre小鼠转基因中含有这两种基因 已赞过 已踩过 你对这个回答的评价是? http://www.informatics.jax.org/allele/MGI:3053818 diamondback rattlesnake florida map https://centreofsound.com

JCI - Osteoclast-specific cathepsin K deletion stimulates S1P …

WebDec 13, 2008 · 成骨细胞特异性表达Cre重组酶转基因小鼠. 含有骨钙素基因启动子、Cre重组酶基因和人生长激素基因polyA的转基因载体pOC-Cre,以显微注射的方法将4.6 kb的转基因片段OC-Cre导入小鼠受精卵。. 16只子代小鼠中经PCR和Southern杂交鉴定,有2只小鼠携带外源基因,整合率为12.5% ... WebAug 1, 2024 · Cre 工具鼠的种类及应用 Cre 工具鼠主要分为 3 类:全身性 Cre、组织特异性 Cre 和配体或药物诱导 Cre。 全身性 Cre:通过诱导剂来调节驱动 Cre 重组酶的启动子活 … WebIt should also be noted that the reduced expression of Ctsk in Syk ΔOC samples is likely partially due to the inactivation of one of the two alleles of the Ctsk gene by the Ctsk-Cre (Ctsk Cre/+) mutation present in those cells. Taken together, gene expression data indicate a role for Syk in regulation of osteoclast-specific gene expression. diamondback rattlesnake information

LysMcre小鼠是什么 - 百度知道

Category:造血系统常用Cre转基因小鼠及其应用 - ResearchGate

Tags:Ctsk-cre小鼠

Ctsk-cre小鼠

(PDF) Transgenic Mice for a Tamoxifen-Induced ... - ResearchGate

Web6 脑组织特异Cre 转基因小鼠(CaMK2-Cre) 7 星形神经胶质特异Cre 转基因小鼠(GFPA-Cre) 8 Nestin 启动子驱动Cre 转基因小鼠(Nestin-Cre) 9 诱导性全身表达Cre 转基因 … Web030323 STOCK Fos tm2.1(icre/ERT2)Luo /J Fos 2A-iCreER knock-in (also called Fos 2A-iCreERT2 or "TRAP2") was designed to have expression of a tamoxifen-inducible, improved Cre recombinase ( icre/ERT2 ) from the Fos promoter/enhancer elements - without disrupting endogenous Fos expression.

Ctsk-cre小鼠

Did you know?

Web由于 Cre 的非特异性表达导致基因敲除的脱靶,有可能在你不自知的时候直接影响到你的实验数据。. 比如,受神经元特异性 Emx1(empty spiracles homolog 1)启动子驱动的 Cre 小鼠,除了在新皮质和海马中高表达 Cre 以外,在肾脏和胸腺中也会表达 Cre。. … WebMay 18, 2012 · Other CtsK-Cre lines have also been reported that are not knock-in lines, but based on Ctsk-Cre transgenic overexpression [50, 51]. These have been used for deletion of Fcgr4 [52], Vdr [53], and ...

WebMar 13, 2024 · 近期,骨膜干细胞的神秘面纱终于被揭开,来自于美国康奈尔大学威尔医学院的Matthew B. Greenblatt研究团队在Nature杂志发表了题为Discovery of a periosteal stem cell mediating intramembranous bone formation的研究快报,首次发现了骨膜干细胞并鉴定了其内成骨的特性,解决了困扰 ... Web三、鉴定Cre鼠删除效率. 纯合或杂合flox鼠与Cre鼠交配的后代需要鉴定删除效率,验证是否发生删除。验证删除效率前需要先鉴定Cre基因,若鼠尾为Cre阳性再取特异性组织进行验证。 1. 引物设计 . 在5'arm与3'arm上设计引物,位于cKO区域的上/下游。 2. 预期片段大小

WebCre基因前,使其启动Cre酶的表达,从而介导lox 位点问特定基因DNA片段的敲除,当敲除过程完成 后,DNA片段重新结合,形成一个lox位点。 http://www.larc.tsinghua.edu.cn/post/971/31

WebJan 16, 2013 · To analyze the effect of targeted deletion of Ctsk in the osteoblast lineage, 13-week-old Osx-Cre;Ctsk +/+ and Osx-Cre;Ctsk fl/fl mice were fluorochrome injected on days 9 and 2 before necropsy. Mice were killed by cardiectomy. Femurs were removed and fixed in 70% alcohol. They were dehydrated in ethanol, infiltrated, and embedded without ...

Web根据巨噬细胞定居的组织不同,可将其分为肝脏中的Kupffer细胞、脑中的小胶质细胞、骨组织中的破骨细胞等,不同组织的巨噬细胞具有不同的marker 基因,因此需要选择不同的 … diamondback rattlesnake headcircle of willis diagram labeledWebCathepsin K-Cre, CatK Cre, Ctsk Cre, CtsKCre: Gene: Ctsk Location: Chr3:95406567-95416673 bp, + strand Genetic Position: Chr3, 40.74 cM Alliance: Ctsk tm1(cre)Ska … diamondback rattlesnake imagesWebMay 9, 2024 · 今天看到一段对Cre+/-,flox/flox的描述,受益匪浅,摘录下来,以备后用。 Cre+/-,flox/flox表示携带1个Cre基因的条件剔除小鼠 Cre ... circle of willis labelingWebCtsk-Cre小鼠与FloxP小鼠交配后,在Cre特异表达的组织或者器官或细胞中,两个FloxP位点间的外显子或元件将被删除,达到条件性敲除或过表达的目的。可用于骨骼肌系统等相 … diamondback rattlesnake lengthWebCtsk-cre: Transgene: Tg(Ctsk-cre)1Rda Location: unknown Alliance: Tg(Ctsk-cre)1Rda page Transgene origin: Strain of Origin: C57BL/6 or (C57BL/6 x CBA)F1 Transgene … circle of willis blood flow directionWeb活体. NM-KI-18039. Cdkn2a-Luc-2A-tdTomato-2A-CreERT2. 其他. 衰老细胞;肿瘤细胞. 活体. * 列表中存在部分Dre工具鼠,需要与Frox(Flanked by Rox sites,目的基因或序列被Rox位点分隔)小鼠杂交繁育,获得目的小鼠。. circle of willis label quiz